logic, and the sexuality of plants was once more openly impugned in the face of Koelreuter's investigations. This state of
things continued till some time after 1820, but then it began
to improve once more. L. C. Treviranus examined and
refuted the errors of Schelwer and Henschel in 1822; in England Herbert conducted new and very valuable investigations
into the question of hybridisation; and it was in this period
that Carl Friedrich Gärtner studied and experimented on
normal fertilisation and the production of hybrids during more
than twenty years; his conclusions, published in exhaustive works in 1844 and in 1849, finally settled the more important
questions connected with the sexual theory about the same
time that Hofmeister established the microscopic embryology
of Phanerogams on a firm foundation.
Other parts also of vegetable physiology had been considerably advanced before 1840; Theodore de Saussure observed in 1822 the production of heat in flowers and its dependence on respiration ten years later Goeppert proved the rise of temperature in germinating and vegetating organs. Dutrochet stimulated enquiry by his researches in various branches of the science between 1820 and 1840; he was the first to apply the phenomena of diosmosis to the explanation of the movement of sap in plants with a lasting influence on the further progress of physiology. Chemical investigations were less fruitful in results, though they served to collect a considerable material of single facts, which could afterwards be turned to theoretical account.
The close of this period, which began with unprofitable doubts, but in which much was set in a train for further development after 1840, is marked by the publication of some important compilations, in which all that had as yet been done in vegetable physiology was presented in a connected form. In addition to Dutrochet's collected works (1837) three comprehensive compendia of vegetable physiology made their appearance, one by De Candolle, which was translated
