nearly as possible at the same temperature; but I will here and elsewhere give only a few of the many trials made. A leaf was left for 10 m. in water at 100° (37°.7 Cent.), but no inflection occurred. A second leaf, however, treated in the same manner, had a few of its exterior tentacles very slightly inflected in 6 m., and several irregularly but not closely inflected in 10 m. A third leaf, kept in water at 105° to 106° (40°.5 to 40°.1 Cent.), was very moderately inflected in 6 m. A fourth leaf, in water at 110° (43°.3 Cent.), was somewhat inflected in 4 m., and considerably so in from 6 m. to 7 m.
Three leaves were placed in water which was heated rather quickly, and by the time the temperature rose to 115°-116° (46°.1 to 46°.06 Cent.), all three were inflected. I then removed the lamp, and in a few minutes every single tentacle was closely inflected. The protoplasm within the cells was not killed, for it was seen to be in distinct movement; and the leaves, having been left in cold water for 20 hrs., re-expanded. Another leaf was immersed in water at 100° (37°.7 Cent.), which was raised to 120° (48°.8 Cent.) ; and all the tentacles, except the extreme marginal ones, soon become closely inflected. The leaf was now placed in cold water, and in 7 hrs. 30 m. it had partly, and in 10 hrs. fully, re-expanded. On the following morning it was immersed in a weak solution of carbonate of ammonia, and the glands quickly became black, with strongly marked aggregation in the tentacles, showing that the protoplasm was alive, and that the glands had not lost their power of absorption. Another leaf was placed in water at 110 (43°.3 Cent.) which was raised to 120 (48°.8 Cent.) ; and every tentacle, excepting one, was quickly and closely inflected. This leaf was now immersed in a few drops of a strong solution of carbonate of ammonia (one part to 109 of water) ; in 10 m. all the glands became intensely black, and in 2 hrs. the protoplasm in the cells of the pedicels was well aggregated. Another leaf was suddenly plunged, and as usual waved about, in water at 120°, and the tentacles became inflected in from 2 m. to 3 m., but only so as to stand at right angles to the disc. The leaf was now placed in the same solution (viz. one part of carbonate of ammonia to 109 of water, or 4 grs. to 1 oz., which I will for the future designate as the strong solution), and when I looked at it again after the interval of an hour, the glands were blackened, and there was well-marked aggregation. After an additional interval of 4 hrs, the tentacles had become much more inflected. It deserves notice that a solution as strong as this never causes inflection in ordinary cases. Lastly, a leaf was suddenly placed in water at 125 (51.0 Cent.), and was left in it until the water cooled; the tentacles were rendered of a bright red and soon became inflected. The contents of the cells underwent some degree of aggregation, which in the course of three hours increased ; but the masses of protoplasm did not become spherical, as almost always occurs with leaves immersed in a solution of carbonate of ammonia.
We learn from these cases that a temperature of from
120° to 125° (48°.8 to 51°.6 Cent.) excites the tentacles
