I have shown {Expt. 23) that a detached branch of Mimosa can be kept alive for several days with the cut end immersed in water. In this condition the pulvi- nus retains its sensitiveness for more than two days. The excitability undergoes a continuous decline and is abolished about the fiftieth hour. Isolation from the parent organism thus causes a continuous depression of the tonic condition of the specimen. The case is somewhat analogous to the depression of excitability in an excised bloodless muscle. It is thus possible to secure specimens of varying degrees of sab-tonicity. A specimen that has been detached for six hours will exhibit a slight amount of depression, while a different specimen isolated for twenty- four hours will occupy a very much lower position in the scale of tonicity.
Experiment 49. — The staircase response of Mimosa given in figure 53 was obtained with the stimulus of induction shock. In order to establish a wider generalisa- tion I now used the stimulus of light given by an arc lamp. There may bo a difificulty on account of the diurnal movefmsnt of Mimosa ; the leaf, generally speaking, has a movement in a downward dii'ection from morning till noon, after which there is a comparative state of rest. It is better to choose the time of noon for experi- ment. In any case the response to stimulus is very abrupt and in strong contrast with the slow diurnal move- ment A horizontal pencil of light was thrown upwards by means of a small mirror and made to fall on the lower half of a pulvinus of the Mimosa leaf. The excita- tory down moveniiut is f olio we i by recovery on the cessation of light. The intensity of stimulus can be modified by varying the intensity of light. I took for my first series of experiments a specimen that had been isolated for six hours. Stimulation was caused by suc- cessive applications of light for 25 seconds at intervals of
