Page:OJ L No. 65 of 2016 - EN English.pdf/60

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L 65/58
EN
Official Journal of the European Union
11.3.2016

ANNEX

Identification number of the additive Name of the holder of authorisation Additive Composition, chemical formula, description, analytical method Species or category of animal Maximum age Minimum content Maximum content Other provisions End of period of authorisation
Units of activity/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: digestibility enhancers.
4a16 Huvepharma EOOD 6-phytase (EC 3.1.3.26) Additive composition
Preparation 6-phytase (EC 3.1.3.26) produced by Komagataella pastoris (DSM 23036) with a minimum activity of:
4 000 OTU ([1])/g in solid form
8 000 OTU/g in liquid form
Characterisation of the active substance
6-phytase (EC 3.1.3.26) produced by Komagataella pastoris (DSM 23036)
Analytical method([2])
Colorimetric method based on the quantification of the inorganic phosphate released by the enzyme from the sodium phytate
Chickens for fattening, chickens reared for laying, laying hens, other avian species other than turkeys for fattening and turkeys reared for breeding, pigs for fattening, sows. 125 OTU

1. In the directions for use of the additive and premixture, indicate the storage temperature, storage life, and stability to pelleting.
2. Recommended maximum dose for all authorised species: 500 OTU/Kg of complete feedingstuff.
3. For use in feed containing more than 0,23 % phytin-bound phosphorus.
4. For safety: breathing protection, glasses and gloves shall be used during handling.

28 February 2022
Turkeys for fattening, turkeys reared for breeding, piglets (weaned) 250 OUT
  1. 1 OTU is the amount of enzyme that catalyzes the release of 1 micromole of inorganic phosphate per minute from 5,1 mM sodium phytate in pH 5,5 citrate buffer at 37 °C, measured as the blue P-molybdate complex color at 820 nm.
  2. Details of the analytical methods are available at the following address of the Community Reference Laboratory: http://irmm.jrc.ec.europa.eu/EURLs/EURL_feed_additives/Pages/index.aspx