ease is so common in some districts, in which its spread can not be ascribed to the bite of flies. Grass, on which the germs of bacteridia had been placed, was given to the sheep. A certain number of them died of splenic fever. The glands and tissues of the back of the throat were very much swelled, as if the inoculation had occurred in the upper part of the alimentary canal, and by means of slight wounds on the surface of the mucous membrane of the mouth. In order to verify the fact, the grass given to the sheep was mixed with thistles and bearded ears of wheat and barley, or other prickly matter, and in consequence the mortality was sensibly increased.
In cases of spontaneous disease it was surmised that the germs which were artificially introduced into food in the course of these experiments are found upon the grass, especially in the neighborhood of places in which infected animals had been buried. It was, in fact, ascertained that these germs existed above and around the infected carcasses, and that they were absent at a certain distance from their burial-place. It is true that putrid fermentation destroys most of the bacteria, but before this occurs a certain number of microbes are dispersed by the gas disengaged from the carcass; these dry up and produce germs, which retain their vitality in the soil for a long while.
The mechanism by means of which these germs are brought to the surface of the soil and on to the grass on which the sheep feed is at once simple and remarkable. Earth-worms prefer soils which are rich in humus or decomposing organic substance, and seek their food round the carcass. They swallow the earth containing the germs of which we have spoken, which they deposit on the surface of the soil, after it has traversed their intestinal canals, in the little heaps with which we are all acquainted. The germs do not lose their virulence in their passage through the worms' intestines, and, if the sheep swallow them together with the grass on which they browse, they may contract the disease. The turning-up of the soil by the spade or plow may produce the same effect.
A certain warmth is necessary for the formation of germs; none are produced when it falls below 12°, and the carcasses buried in winter are therefore less dangerous than those buried in the spring and summer. It is, in fact, in hot weather that the disease is most prevalent. Animals may, however, contract it even in their stalls from eating dry fodder on which germs of these bacteria remain.
Pasteur and his pupils performed an experiment in the Jura in 1879, which clearly shows that the presence of germs above the trenches in which carcasses have been buried is the principal cause of inoculation. Twenty oxen or cows had perished, and several of them were buried in trenches in a meadow where the presence of these germs was ascertained. Three of the graves were surrounded by a fence, within which four sheep were placed. Other sheep were folded within a few yards of the former, but in places where no infected animals had been buried.
