The vaccine thus obtained in Pasteur's laboratory is now distributed throughout the world, and has already saved numerous flocks from almost certain destruction. Although this process has only been known for a few years, its results are such that the gain to agriculture already amounts to many thousands of pounds.
Toussaint makes use of a slightly different mode of preparing a vaccine virus, which is, however, analogous to that of Pasteur. He subjects the lymph of the blood of a diseased animal to a temperature of 50°, and thus transforms it into vaccine. Toussaint considers the high temperature to be the principal agent of attenuation, and ascribes little or no importance to the action of the oxygen in the air.
Chamberland and Roux have recently made researches with the object of obtaining a similar vaccine by attenuating the primitive virus by means of antiseptic substances. They have ascertained that a solution of carbolic acid of one part in six hundred destroys the microbes of anthrax, while they can live and flourish in a solution of one part in nine hundred, but without producing spores, and their virulence is attenuated. When a nourishing broth is added to a solution of one in six hundred, the microbe can live and grow in it for months. Since the chief condition of attenuation consists in the absence of spores, this condition seems to be realized by the culture in a solution of carbolic acid, one in nine hundred, and it is probable that a fresh form of attenuated virus may thus be obtained. Diluted sulphuric acid gives analogous results. However this may be, the vaccine prepared by Pasteur's process is the only one which has been largely used, and which has afforded certain results to cattle-breeders.
Public experiments, performed before commissions composed of most competent men, have clearly shown the virtue of the protective action. In the summer of 1881 the initiation was taken by the Melun Society of Agriculture. Twenty-five sheep and eight cows or oxen were vaccinated at Pouilly-le-Fort, and then reinoculated with blood from animals which had recently died of anthrax, together with twenty-five sheep and five cows which had not been previously vaccinated. None of the vaccinated animals suffered, while the twenty-five test sheep died within forty-eight hours, and the five cows were so ill that the veterinary surgeons despaired of them for several days.
This experiment was publicly repeated in September, 1881, by Thuillier, Pasteur's fellow-worker, whose death we have recently had to deplore, before the representatives of the Austro-Hungarian Government; and again near Berlin, in 1882, before the representatives of the German Government, and always with the same success. Up to April, 1882, more than one hundred and thirty thousand sheep and two thousand oxen or cows had been vaccinated; and since that time the demand for vaccine from Pasteur's laboratory has reached him from every quarter.
