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since confirmed by many others. This proof consists in the experimental inoculation of healthy individuals with blood containing the parasite and the development of a typical attack of periodic fever as a result of such inoculation. Marchiafava and Bignami, in their elaborate article upon 'Malaria' published in the 'Twentieth Century Practice of Medicine' say:

"The transmission of the disease occurs equally whether the blood is taken during the apyretic period or during a febrile paroxysm, whether it contains young parasites or those in process of development, or whether it contains sporulation forms. Only the crescent forms, when injected alone, do not transmit the infection, as has been demonstrated by Bastianelli, Bignami and Thayer, and as can be readily understood when we remember the biological significance of these forms.

"In order that the disease be reproduced in the inoculated subject it is not necessary to inject the malarial blood into a vein of the recipient, as has been done in most of the experiments; a subcutaneous injection is all-sufficient. Nor is it necessary to inject several cubic centimeters, as was done especially in the earlier experiments; a fraction of a cubic centimeter will suffice and even less than one drop, as Bignami has shown."

After the inoculation of a healthy individual with blood containing the parasite a period varying from four to twenty-one days elapses before the occurrence of a febrile paroxysm. This is the so-called period of incubation, during which, no doubt, the parasite is undergoing multiplication in the blood of the inoculated individual. The duration of this period depends to some extent upon the quantity of blood used for the inoculation and its richness in parasites. It also depends upon the particular variety of the parasite present, for it has been ascertained that there are at least three distinct varieties of the malarial parasite—one which produces the quartan type of fever, in which there is a paroxysm every third day and in which, in experimental inoculations made, the period of incubation has varied from eleven to eighteen days; in the tertian type, or second day fever, the period of incubation noted has been from nine to twelve days; and in the æstivo-autumnal type the duration has usually not exceeded five days. The parasite associated with each of these types of fever may be recognized by an expert, and there is no longer any doubt that the difference in type is due to the fact that different varieties or 'species' of the malarial parasite exist, each having a different period of development. Blood drawn during a febrile paroxysm shows the parasite in its different stages of intra-corpuscular development. The final result of this development is a segmenting body, having pigment granules at its center, which occupies the greater part of the interior of the red corpuscle. The number of segments into which this body divides differs in the different types of fever, and there are other points of difference by which the several varieties may be distinguished one from the other, but which it is not necessary to mention