every instance where this can be done it is perfectly clear that these chromosomes do not fuse together nor lose their identity, but that every chromosome splits lengthwise and its halves separate and go into the two daughter cells where they form the daughter nuclei. Each of these cells therefore receives half of its chromosomes from the egg and half from the sperm. Even in cases where the individual chromosomes are lost to view in the daughter nuclei those nuclei may be clearly double, one half of each having come from the egg chromosomes and the other half from the sperm chromosomes (Fig. 26 B).
At every subsequent cleavage of the egg the chromosomes divide in exactly the same way as has been described for the first cleavage. Every cell of the developing animal receives one half of its chromosomes from the egg and the other half from the sperm, and if the chromosomes of the egg differ in shape or in size from those of the sperm, as is sometimes the case when different races or species are crossed, these two groups of chromosomes may still be distinguished at advanced stages of development. Where the egg and sperm chromosomes are not thus distinguishable it may still be possible to recognize the half of the nucleus which comes from the egg and the half which comes from the sperm even up to an advanced stage of the cleavage (Fig. 26).
At the same time that the maternal and paternal chromosomes are being distributed with such precise equality to all the cells of the developing organism, the different substances in the cell body outside of the nucleus may be distributed very unequally to the cleavage cells. The movements of the cytoplasm of the egg which began with the flowing of the surface layer to the point of entrance of the sperm, lead to the segregation of different kinds of plasms in different parts of the egg and to the unequal distribution of these substances to different cells.
One of the most striking cases of this is found in the ascidian, Styela, in which there are four or five different kinds of substance in the egg which differ in color, so that their distribution to different regions of the egg and to different cleavage cells may be easily followed, and even photographed while in the living condition. The peripheral layer of protoplasm is yellow and when it gathers at the lower pole of the egg where the sperm enters it forms a yellow cap. This yellow substance then moves, following the sperm nucleus, up to the equator of the egg on the posterior side and there forms a yellow crescent extending around the posterior side of the egg just below the equator. On the anterior side of the egg a gray crescent is formed in a somewhat similar manner and at the lower pole between these two crescents is a slate blue substance, while at the upper pole is an area of colorless protoplasm. The yellow crescent goes into cleavage cells which become muscle and mesoderm, the gray crescent into cells which become nervous system and notochord, the slate-blue substance into endoderm cells and the colorless substance into ectoderm cells.
