240 The Vaccine Institute, Belgaum.
The vesicles obtained on the rabbit are not formed like the vesicles found on a calf or child and often only a number of raised red papules are obtained. These, however, contain the active virus and must be scraped away with the sharp scalpel. The admixture of some blood in the scrapings does not matter and the material is rubbed up in an agate mortar with 50 per cent glycerine and water. The emulsion is then stored for use, and, when required, is used to vaccinate a calf. The result of the first move, on. the calf, is usually good enough to be used for despatch. The best of the vesicles are chosen and collected for seed for calf vaccination. It is found that, after passage through the rabbit, the vesiculation on the calf is greatly improved, and gives firm, unbroken vesicles after 120 hours.
APPENDIX A.
Statement showing the results obtained with Belgaum lymph during the years 1914-15 to 1918-19 inclusive.
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